Update previously stored list of datetimes in a combobox, in a cycle for

Hi, how can I make sure that all the datetime stored previously by user input will be updated in a cycle for?
I explain better the structure
Combobox2 <----- is where list of datatime are previously stored
Combobox3 <------- is where remaining time is given by a substraction of two dates, stored in days remaing.
The fact, is now that these values are only stored and doesn't change with time passing by.
This is my code

Private Sub Button5_Click(sender As Object, e As EventArgs) Handles Button5.Click

        For i = 0 To ListBox1.Items.Count
            Dim d As DateTime = DateTime.Now
            Dim d2 As DateTime = ComboBox2.SelectedItem(i)
            Dim dysdiff As String = (d - d2).Days.ToString()
            ComboBox3.SelectedItem(i) = 365 - dysdiff & " Days"
        Next
    End Sub